The executive order also entails the U.S. withdrawing from the U.N. Human Rights Council and stopping funds to the United Nations Relief and Works Agency (UNRWA), which delivers vital assistance to millions in Gaza. These actions align with Trump's previous decisions during his first presidential term.

Equally noteworthy, the order calls for a reexamination of U.S. participation in UNESCO over claims of an alleged bias against American interests, which his administration views as unfair in light of the funding disparities between nations. The White House staff secretary, Will Scharf, remarked on these funding inequalities as “deeply unfair to the U.S.”

Following the announcement, U.N. spokesman Stéphane Dujarric emphasized the value of U.S. support for the United Nations in achieving global security and expressed hopes for ongoing productive relations with Trump's administration amid a shifting geopolitical landscape.

The U.N. has been anticipating challenges in its relationship with Washington as Trump embarks on his second term, aiming to avoid public confrontations in light of past tensions during his initial term.